Journal: Cancer Cell International
Article Title: A1, an innovative fluorinated CXCR4 inhibitor, redefines the therapeutic landscape in colorectal cancer
doi: 10.1186/s12935-024-03584-y
Figure Lengend Snippet: The figure illustrates the inhibitory effects of AMD3100 and A1 on CXCL12-induced AKT phosphorylation in CT-26 treated cells. In panel ( A ), representative Western blot images depict the expression of phosphorylated AKT (pAKT) and Actin-β (as a loading control) in cells subjected to 100 ng/mL of CXCL12 alongside increasing concentrations of either AMD3100 or A1 (0, 5, and 10 µM). The treatment with AMD3100 and A1 resulted in a noticeable reduction in pAKT levels compared to the control group. Panels ( B ) and ( C ) quantify the relative pAKT expression normalized to Actin-β for AMD3100 and A1, respectively. In panel ( B ), cells treated with 10 µM AMD3100 exhibited a statistically significant decrease in pAKT levels compared to both the control (***p < 0.001) and the 5 µM groups (**p < 0.01). Conversely, the difference between the control and the 5 µM groups was not statistically significant. In panel (C), A1 treatment demonstrated a significant reduction in pAKT levels in both the 5 µM (****p < 0.0001) and 10 µM (***p < 0.001) groups in comparison to the control, with the 10 µM group exhibiting a further reduction relative to the 5 µM group (****p < 0.0001). Statistical analysis was performed utilizing one-way ANOVA followed by Tukey’s post hoc test, and the data are presented as mean ± SEM from three independent experiments
Article Snippet: Subsequently, proteins were separated by SDS-PAGE electrophoresis, transferred onto membranes, and immunoblotted with mouse anti-pAKT antibodies (R&D System, MAB887-SP) along with anti-actin β antibodies (R&D System, MAB8929-SP) for normalization.
Techniques: Phospho-proteomics, Western Blot, Expressing, Control, Comparison